Merck, MAK130-1KT, Chromium Assay Kit

General description
Chromium, an essential trace element, is a transition metal that primarily exists in two stable oxidation states, hexavalent [Cr(VI)] and trivalent [Cr(III)]. Chromium is widely used in various industries such as electroplating, leather tanning, chrome paint, dying, hardened steel, ceramic, and glass. Cr(VI) is produced almost exclusively by industrial processes; whereas, in nature chromium primarily exists in its trivalent form. Cr(III) is generally regarded as nontoxic due to its poor absorption. Cr(VI) is considered a pulmonary carcinogen and has tested positive in genotoxicity tests. Cr(VI) is considered a serious pollutant in many water streams due to its carcinogenic potential. In trace amounts, Cr(III) may be required for glucose and lipid metabolism.
Application
This kit is suitable for the detection of chromium in biological, environmental, food, and beverage samples.
Biochem/physiol Actions
In this kit, chromium is determined in a simple, one-step assay in which Cr(VI) forms a specific complex with a chromgenic dye, resulting in a colorimetric (480 nm) product, proportional to the chromium present. Cr(III) can be converted to Cr(VI) with nitric acid/hydrochloric acid, allowing the determination of Cr(III) and/or total chromium [Cr(III) +Cr(VI)].
Features and Benefits
Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.