Merck, MAK152-1KT, Colorimetric Sphingomyelinase Assay Kit

General description
Sphingomyelinase (SMase), or sphingomyelin phosphodiesterase, catalyzes the hydrolytic cleavage of sphingolipid to phosphocholine and ceramide, a second messenger involved in multiple cellular responses including the cellular response to stress. There are at least 5 types of SMases identified which are classified according to their cation dependence, cellular location, and pH optima for activity and include the acidic and neutral sphingomyelinase. Neutral SMases, along with the acidic SMases, are thought to be major contributors to the production of ceramide in response to cellular stress.
Application
This kit is suitable for the detection of sphingomyelinase activity in a variety of samples such as blood and cell extracts or for screening sphingomyelinase inhibitors and activators.
Biochem/physiol Actions
The Sphingomyelinase Assay Kit provides a simple and direct procedure for measuring neutral SMase activity in a variety of samples such as blood or cell extracts, or for screening SMase activators and inhibitors. SMase activity is determined by a coupled enzyme assay, which results in a colorimetric (655 nm) product proportional to the SMase activity present.
Features and Benefits
Compatible with high-throughput handling systems.