Merck, MAK306-1KT, LCFC-LCAT Acyltransferase Activity Assay

General description
The plasma protein lecithin:cholesterol acyltransferase (LCAT) catalyzes the transfer of an acyl group from the sn2 position of phosphatidylcholine to the 3-hydroxyl group of cholesterol forming cholesteryl ester. This activity occurs on the surface of high density lipoprotein (HDL) and the cholesteryl esters formed by LCAT are incorporated into the core of HDL.
Application
Suitable for high-throughput screening, mechanism of action studies and structureactivity relationship (SAR) work of LCAT in plasma and serum
Biochem/physiol Actions
The LCFC-LCAT Acyltransferase Activity Assay is a fluorometric assay useful for measuring the acyltransferase activity of LCAT in serum or plasma. The method detects changes in LCAT free cholesterol (LCFC) levels in the sample without the use of cholesterol oxidase, peroxidase, or the generation of hydrogen peroxide. Detection is not affected by iodoacetate or other LCAT inhibitors. LCAT activity results in a fluorometric (λEx = 320 nm/λEm = 405 nm) product proportional to the amount of free cholesterol present.
Features and Benefits
Non-enzymatic determination of cholesteryl ester formation.
Not affected by LCAT inhibitors or other chemical compounds.
Dilution or non-dilution type assay.
Assay components stable for up to one year.
Compatible with high-throughput handling systems.