Description
General description
Nitric oxide (NO) is a highly reactive radical that plays a crucial role in numerous essential physiological functions. It is produced by the oxidation of arginine through nitric oxide synthase (NOS), and it actively participates in various processes, including host defense, activation of regulatory proteins, and direct covalent interactions with functional biomolecules. Inhibition of NOS can have diverse biological effects, particularly within the cardiovascular system. Simple, direct and non-radioactive procedures for measuring NOS are becoming popular in drug discovery, biomedical and biochemical research.
Application
Suitable for inhibitor screening and evaluation of nitric oxide synthase inhibitors.
The Nitric Oxide Synthase Screening Kit may be used for:
Drug Discovery
Cardiovascular Research
Immunology Research
Biochem/physiol Actions
The Nitric Oxide Synthase Inhibitor Screening Kit involves two steps: a NOS reaction step during which NO is produced followed by an NO detection step. Since the NO generated by NOS is rapidly oxidized to nitrite and nitrate, the NO production is measured following reduction of nitrate to nitrite using an improved Griess method.
The mechanism of action for the Nitric Oxide Synthase Inhibitor Screening Kit comprises two steps:
NOS Reaction Step: In this step, nitric oxide (NO) is produced through the activity of nitric oxide synthase (NOS).
NO Detection Step: Following NO generation, the produced NO undergoes rapid oxidation to form nitrite and nitrate. The measurement of NO production is then conducted by quantifying the reduction of nitrate to nitrite, employing an enhanced Griess method.
Features and Benefits
Efficient Inhibitor Screening: Screen for NOS inhibitors with only 5 µL of test compound, offering a streamlined and effortless procedure.
High-Throughput Compatibility: Seamlessly integrate our kit into high-throughput handling systems, enhancing efficiency and accuracy in screening processes.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
