Description
General description
Glutathione (GSH), composed of the amino acids glycine, glutamic acid, and cysteine, serves as a fundamental antioxidant within the body, playing a pivotal role in cellular defense against oxidative stress caused by reactive oxygen species (ROS). As the major free thiol in cells, GSH is instrumental in various biological processes, including the detoxification of harmful substances, the removal of hydroperoxides, and the maintenance of protein sulfhydryl groups in their reduced state. GSH achieves this by directly reducing disulfide bonds in cytoplasmic proteins to cysteines, during which it is transformed into its oxidized form, glutathione disulfide (GSSG). This cycle between reduced and oxidized forms is critical for maintaining the cellular redox balance.Glutathione functions as a cofactor for several key antioxidant enzymes, enhancing their ability to neutralize free radicals. It also plays a crucial role in the regeneration of vitamins C and E, thereby contributing to their antioxidative effects. Beyond its antioxidant properties, GSH is vital for mitochondrial function and the integrity of mitochondrial DNA (mtDNA), which is essential for energy production and cellular health. Dysregulation of glutathione levels is associated with the pathogenesis of several neurodegenerative diseases such as Alzheimer′s, Parkinson′s, and Huntington′s diseases, as well as other health conditions including cystic fibrosis, immune disorders, and cardiovascular diseases. Glutathione′s role in metabolism and cellular protection is extensive, influencing cellular proliferation, apoptosis, and overall physiological stability.
Application
The Glutathione GSH/GSSG Assay Kit may be used for:
Cancer Research
Diabetes Research
Neurodegenerative Disease Research
The kit is suitable for the quantitative determination of reduced and oxidized glutathione (GSH/GSSG) in whole blood, plasma, serum, urine, tissue and cell extracts.
Biochem/physiol Actions
The Glutathione GSH/GSSG Assay Kit is designed to accurately measure total, reduced and oxidized glutathione in biological samples using an enzymatic method that utilizes Ellman′s Reagent (DTNB) and glutathione reductase (GR). DTNB reacts with reduced glutathione to form a yellow product. The rate of change in the optical density, measured at A412 nm, is directly proportional to the glutathione concentration in the sample. This kit can also be used to measure oxidized (GSSG) by using a specific protocol which first scavenges all GSH with 1-methyl-2-vinylpyridinium triflate.
Features and Benefits
Sensitive Detection Range: Detect Glutathione levels accurately across a wide range, from 0.01 µM to 3 µM, using only 25 μL of sample in a 96-well plate setup, ensuring highly sensitive analysis with minimal sample requirement.
Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 10 minute room temperature reaction, reducing complexity and saving valuable time and effort.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Other Notes
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