Merck, MAK442-1KT, Sorbitol Assay Kit

General description
Sorbitol, also known as glucitol, is a sugar alcohol metabolized slowly within the human body. It is derived from glucose through the reduction of an aldehyde group to a hydroxyl group. Excessive accumulation of sorbitol in erythrocytes, retinal cells, and Schwann cells has been linked to various health issues including retinopathy, cataracts, peripheral neuropathy, and diabetes. Sorbitol is primarily sourced from corn syrup and naturally occurs in fruits such as apples, pears, peaches, and prunes. Widely utilized as a sugar substitute and laxative, sorbitol also finds applications in specialty culture media, as well as in healthcare, food, and cosmetic products. Monitoring sorbitol levels in biological samples aids in understanding metabolic pathways and tracking the progression of diabetes.
Application
The Sorbitol Assay Kit may be used for:
Metabolism Research
Food and Beverage Analysis
Diabetes Research

The kit is suitable for the quantitative determination of D-sorbitol and evaluation of drug effects on sorbitol metabolism in biological (e.g. blood), food, beverage and agriculture samples.
Biochem/physiol Actions
The sorbitol assay involves an end-point enzyme coupled MTT/NAD reaction that forms a colored product with an absorption maximum at A565 nm. The increase in absorbance at A565 nm is directly proportional to the sorbitol concentration.
Features and Benefits
Fast and Precise Measurement: Achieve results in less than 1 hour, providing rapid analysis with a detection range of 5 - 1000 μM D-sorbitol ensures accurate measurements across a wide range of concentrations.
High-Throughput Compatibility: Effortlessly integrate our kit into high-throughput handling systems, optimizing efficiency and ensuring accurate results.

Other Notes
For additional information on our range of Biochemicals, please complete this form.