Merck, MAK451-1KT, Glucose-6-Phosphate Dehydrogenase Assay Kit

General description
Glucose-6-Phosphate Dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia.
Application
The Glucose-6-Phosphate Dehydrogenase Assay Kit may be used for:
Metabolism Research
Drug Discovery

The kit is suitable for the quantitative determination of Glucose-6-Phosphate concentration and evaluation of drug effects on its metabolism in plasma, serum, tissue and culture media, etc.
Biochem/physiol Actions
The non-radioactive, colorimetric Glucose-6-Phosphate Dehydrogenase Assay Kit is based on the reduction of tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A565 nm. The increase in absorbance at A565 nm is proportional to the enzyme activity.
Features and Benefits
Efficient and Precise: Requires only 20 μL of sample and a short 15-minute reaction time while detecting glucose-6-phosphate dehydrogenase levels across a wide range from 0.2 to 100 U/L, offering both convenience and sensitivity.
Compatibility with High-Throughput Systems: Effortlessly incorporate our kit into high-throughput handling systems, guaranteeing seamless integration and maintaining efficiency and accuracy in your laboratory operations.

Other Notes
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