Merck, MAK461-1KT, pNPP Phosphatase Assay Kit

General description
Para-nitrophenyl phosphate (pNPP) is a chromogenic substrate for most phosphatases such as alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The reaction yields para-nitrophenol, which becomes an intense yellow soluble product under alkaline conditions and can be conveniently measured at A405 nm on a spectrophotometer.
Application
The kit is suitable for the quantitative determination of phosphatase activity of protein phosphatases.
The pNPP Phosphatase Assay Kit may be used for:
Metabolism Research
Cardiovascular Research
Neurodegenerative Research

Biochem/physiol Actions
The pNPP Phosphatase Assay kit involves adding a single reagent to the phosphatase and measuring the product formation on an absorbance reader. The assay can be conveniently performed in cuvettes, tubes or multi-well plates at either room temperature or 37 °C.
Features and Benefits
Simplified Process: Enjoy a hassle-free procedure that can be completed within 30 minutes without wash or reagent transfer steps, saving you time and effort.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Other Notes
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