Merck, MAK465-1KT, Glycogen Assay Kit

General description
Glycogen is a branched polysaccharide of glucose units linked by α-1,4 glycosidic bonds and α-1,6 glycosidic bonds. It is stored primarily in the liver and muscle and forms an energy reserve that can be quickly mobilized to meet a sudden need for glucose. The most common glycogen metabolism disorder is found in diabetes, in which liver glycogen can be abnormally accumulated or depleted due to abnormal amounts of insulin. Genetic glycogen storage diseases have been associated with various inborn errors of metabolism caused by deficiencies of enzymes necessary for glycogen synthesis or breakdown.
Application
The kit is suitable for the quantitative determination of glycogen in biological samples such as tissue and cells, as well as the evaluation of drug effects on glycogen metabolism.
Biochem/physiol Actions
The Glycogen Assay Kit uses a single working reagent that combines the enzymatic break down of glycogen and the detection of glucose in one step. The color intensity of the reaction product at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the glycogen concentration in the sample.
Features and Benefits

• Linear detection range: 2 to 200 µg/mL glycogen for colorimetric assays and 0.2 to 20 µg/mL for fluorometric assays.
• Convenient room temperature assay. The procedure involves adding a single working reagent, and takes only 30 minutes.