Merck, MAK467-1KT, Aspartate Transaminase (AST) Assay Kit

General description
Aspartate Transaminase (AST), also known as serum glutamic oxaloacetic transaminase (GOT) or aspartate aminotransferase (ASAT/AAT), facilitates the conversion of aspartate and α-ketoglutarate to oxaloacetate and glutamate. There are two isoenzymes in humans: GOT1 is a cytosolic isoenzyme derived from red blood cells and heart; GOT2 is the mitochondrial isoenzyme found mainly in the liver. AST is elevated in liver and muscle diseases. It is part of diagnostic testing for liver function, myocardial infarction, acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, and trauma.
Application
The kit is suitable for AST activity determination in serum, plasma, and other biological samples, as well as for studying the effects of drugs on AST activity.
Biochem/physiol Actions
The Aspartate Transaminase (AST) Assay Kit is based on the quantification of oxaloacetate produced by AST. In this assay, oxaloacetate and NADH are converted to malate and NAD+ by the enzyme malate dehydrogenase. The decrease in NADH absorbance at 340 nm is proportionate to AST activity.
Features and Benefits

• Linear detection range: 2 to 100 U/L
• This simple, convenient assay can be carried out in a microplate or a cuvette and takes only 10 minutes