Merck, MAK472-1KT, Iron Assay Kit

General description
Iron level in blood is a reliable diagnostic indicator of various disease states. Increased levels of iron concentration in blood are associated with red blood cell destruction, decreased blood cell survival, acute hepatitis, certain sideroblastic anemias, ingestion of iron-rich diets, defects in iron storage (e.g., pernicious anemia). Decreased levels of blood iron may result from insufficient iron ingestion from diets, chronic blood loss pathologies, or increased demand on iron storage as during normal pregnancy.
Application
The kit is suitable for the detection of iron in biological samples such as serum and soil extracts, and for studying the effects of drugs on iron metabolism.
Biochem/physiol Actions
The Iron Assay Kit is designed to measure total iron directly in serum without pretreatment. The improved method utilizes a chromogen that forms a blue colored complex specifically with Fe2+. Fe3+ in the sample is reduced to Fe2+, allowing the assay to determine total iron concentration. The intensity of the color, measured at 590 nm, is directly proportional to the iron concentration in the sample.
Features and Benefits

• Linear detection range 27 µg/dL (4.8 µM) to 1,000 µg/dL (179 µM) iron in 96-well plate assay.
• Compatible with high-throughput handling systems.
• The optimized formulation has greatly enhanced reagent and signal stability.
• Low interference in biological samples.
• No pretreatments are needed. Assays can be directly performed on serum samples