Merck, MAK477-1KT, Calcium Assay Kit

General description
Calcium is measured to monitor diseases of the bone or calcium regulation disorders. Increased calcium levels in serum are reported in hyperparathyroidism, metastatic bone lesions and hypervitaminosis. While decreased levels are observed in hypoparathyroidism, nephrosis, rickets, steatorrhea, nephritis and calcium-losing syndromes. Urinary calcium levels aid in understanding how the kidneys handle calcium in certain diseases of the parathyroid gland. Urinary calcium levels are also essential in the evaluation of kidney stones.
Application
The kit is suitable for calcium determination in blood, urine, saliva and other biological samples, as well as for studying the effects of drugs on calcium metabolism.
Biochem/physiol Actions
This Calcium Assay Kit is designed to measure calcium directly in biological samples without any pre-treatment. Phenolsulphonephthalein dye in the kit forms a very stable blue colored complex specifically with free calcium. The intensity of the color, measured at 612 nm, is directly proportional to the calcium concentration in the sample. The optimized formulation minimizes any interference by substances such as magnesium, lipids, protein, and bilirubin.
Features and Benefits

• The linear detection range of the kit is 0.08 mg/dL (20 µM) to 20 mg/dL (5 mM).
• Compatible with high-throughput handling systems.
• The optimized formulation has greatly enhanced reagent and signal stability.
• Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipids, protein, magnesium, iron and zinc.