Merck, MAK479-1KT, NADP/NADPH Assay Kit

General description
Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+ /NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue.
Application
The kit is suitable for detection of NADP+ /NADPH concentrations and ratios in cell or tissue extracts.
Biochem/physiol Actions
The NADP/NADPH Assay Kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportionate to the NADP+ /NADPH concentration in the sample. This assay is highly specific for NADP+ /NADPH and is not interfered by NAD+ /NADH.
Features and Benefits

• The linear detection range of the kit is 0.1 µM to 10 µM NADP+ /NADPH
• Compatible with high-throughput handling systems.
• Convenient, the procedure involves adding a single working reagent, and reading the optical density at time zero and 30 minutes at room temperature.