Merck, MAK486-1KT, Carbonyl Assay Kit

General description
Carbonyl groups, such as ketones and aldehydes, are a common indicator of protein oxidation. Protein oxidation is caused because of exposure to reactive oxygen species (ROS) and is a common marker in various diseases, as well as aging.
Application
The kit is suitable for the quantification of carbonyl groups (e.g. ketones, aldehydes) or protein carbonyls in biological samples (e.g. oxidized BSA, etc.).
Biochem/physiol Actions
The Carbonyl Assay Kit is based on an improved method, where 2,4-dinitrophenylhydrazine (DNPH) reacts with carbonyl groups to produce a colored compound at 375 nm. The intensity of this colored compound is directly proportional to the carbonyl groups in the sample.
Features and Benefits

• Linear detection range from 12 to 250 µM carbonyl
• Compatible with high-throughput handling systems.
• Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes.