Merck, MAK488-1KT, Phosphate Assay Kit

General description
Phosphate (Pi) is one of the most important ion species in nature. Phosphate is present in all biological systems. It is a major constituent in minerals and fertilizers, and is a component of industrial wastewater. Thus accurate determination of phosphate concentration finds numerous applications in pharmacology, biomedical research, clinical chemistry, industrial process monitoring and environmental monitoring.
Application
The kit is suitable for the quantification of phosphate in serum, urine, saliva, sweat, food and beverages, water, soil and fertilizer, as well as studying the effects of drugs on phosphate metabolism.
Biochem/physiol Actions
The Phosphate Assay Kit is designed to measure phosphate ions directly in samples without any pretreatment. The improved Malachite Green method utilizes the malachite green dye and molybdate, which forms a stable colored complex specifically with inorganic phosphate. The intensity of the color, measured at 620 nm, is directly proportional to the phosphate concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
Features and Benefits

• Linear detection range: 0.3 µM (0.0028 mg/dL) to 50 µM (0.47 mg/dL) phosphate.
• Compatible with high-throughput handling systems.
• The optimized formulation has greatly enhanced reagent and signal stability.
• Low interference in biological samples. No pretreatments are needed.