Merck, MAK490-1KT, L-Amino Acid Assay Kit

General description
L-amino acids are essential molecules defined by the nature of their side-chain. There are 20 amino acids that are commonly used by most organisms for the production of proteins and other biologically relevant molecules. While amino acids can occur in either the D- or L-enantimer, only the L-form is used by cells.
Application
The kit is suitable for L-Amino Acid determination in serum, culture media, tissue homogenates, cell lysates, urine, food samples, etc.
Biochem/physiol Actions
The L-Amino Acid Assay Kit uses an enzyme-catalyzed oxidation of L-amino acids to convert a dye into a colored or fluorescent form. The absorbance at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 is directly proportional to the L-amino acid concentration in the sample.
Features and Benefits

• Linear detection range: 3.3 to 500 µM (colorimetric assay) and 0.13 to 50 µM (fluorometric assay) for 60 minute reaction.
• Compatible with high-throughput handling systems.