Merck, MAK493-1KT, Isocitrate Dehydrogenase (IDH) Assay Kit

General description
Isocitrate dehydrogenase (IDH) is an enzyme which catalyzes the interconversion of isocitrate and α-ketoglutarate. There are three IDH isoforms: IDH3 uses the cofactor NAD⁺ and catalyzes the third step in the citric acid cycle, while IDH1 and IDH2 use the cofactor NADP⁺ and catalyze the same reaction outside the citric acid cycle. This kit measures the activity of the IDH1 and IDH2 isoforms. Mutations in IDH1 and IDH2 have been linked with various brain tumors and acute myeloid leukemia.
Application
The kit is suitable for IDH activity determination in biological samples (e.g. plasma, serum, tissue and culture media.)
Biochem/physiol Actions
The Isocitrate Dehydrogenase (IDH) Assay Kit is a non-radioactive, colorimetric IDH assay based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT, which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.
Features and Benefits
Linear detection range 0.1 to 100 U/L for 30 minute reaction.
Compatible with high-throughput handling systems.