Merck, MAK495-1KT, Aspartate Assay Kit

General description
Aspartate, a nonessential amino acid, is a precursor to several other amino acids and is an excitatory neurotransmitter. Aspartate is involved in the urea cycle and gluconeogenesis.
Application
The kit is suitable for aspartate determination in plasma, serum, tissue and culture media, as well as for studying the effects of drugs on aspartate metabolism.
Biochem/physiol Actions
In the Aspartate Assay Kit, aspartate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity of the oxidized dye at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the aspartate concentration in the sample.
Features and Benefits

• Linear detection range: 2 to 400 µM aspartate for the colorimetric assay and 1 to 50 µM for the fluorometric assay.