Merck, MAK509-1KT, Ferric Reducing Antioxidant Power (FRAP) Assay Kit

General description
Ferric Reducing Antioxidant Power (FRAP) is a measure of antioxidant capacity quantified by the antioxidant′s potential to reduce ferric iron (III) to ferrous iron (II). Antioxidants protect cells from damage by reactive oxygen species that are produced by oxidation reactions. As oxidative stress contributes to the development of many diseases including Alzheimer′s disease, Parkinson′s disease, diabetes, rheumatoid arthritis and neurodegeneration, the use of antioxidants in pharmacology is intensively studied. Antioxidants are also widely used as dietary supplements and as preservatives in a wide range of products such as food, cosmetics, rubber and gasoline.
Application
The kit is suitable for the determination of ferric reduction antioxidant potential in plant extracts, foods, vitamins, supplements, and biological samples such as serum, plasma, and urine.
Biochem/physiol Actions
The Ferric Reducing Antioxidant Power (FRAP) Assay Kit measures antioxidant potential in which Fe³⁺ is reduced by antioxidant to Fe²⁺. The resulting Fe²⁺ specifically forms a colored complex with a chromogen. The color intensity at 590 nm is proportional to FRAP in the sample.
Features and Benefits
Linear detection range: 0.5 to 180 µM Fe³⁺ reduction potential
Compatible with high-throughput handling systems.
The optimized formulation has greatly enhanced reagent and signal stability.