Merck, MAK518-1KT, ADP Assay Kit

Name: ADP Assay Kit
Brand: Merck
SKU: MAK518-1KT

Size:1 kit, ADP Detection Kit, sufficient for 100 fluorometric tests

Catalog Number: MAK518-1KT

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Category

BCA Protein Assay Kits

TagsChemistry & Biochemicals Biochemicals Metabolism Assay Kits

CollectionsMerck Biochemicals Collection: Trusted Lab Reagents & Buffers Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Adenosine diphosphate (ADP) is the product of ATP dephosphorylation by ATPases. ADP can be converted back to ATP by ATP synthases. ADP levels regulate several enzymes involved in intermediary metabolism. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, since these assays require measurement of ATP in the sample before conversion of ADP to ATP, if the nascent ATP concentration is significantly higher than the ADP concentration, the ATP signal will drown out the ADP signal.
Application
The kit is suitable for ADP determination in cells and other biological samples.
Biochem/physiol Actions
The ADP Assay Kit provides a convenient fluorometric means to measure ADP level even in the presence of ATP. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate is then quantified by a fluorometric method at λEx = 530 nm/λEm = 590 nm.
Features and Benefits

As low as 0.1 µM ADP can be quantified.
Compatible with high-throughput handling systems.

Specifications

General

NACRES	NA.84
UNSPSC Code	12161503
detection method	fluorometric
relevant disease(s)	hematological disorder
shipped in	wet ice
storage temp.	−20°C
Quality Level	100

Ordering

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