Merck, MAK526-1KT, Diamine Oxidase Assay Kit

General description
Diamine oxidase (DAO) also known as histaminase or amine oxidase (copper containing), is an enzyme involved in the metabolism, oxidation, and inactivation of histamine in animals. Highest levels are observed in the digestive tract and placenta. An imbalance between histamine intake and the capacity for histamine degradation can lead to histamine intolerance (HIT). Measuring DAO activity in serum can be useful in diagnosing HIT.
Application
The kit is suitable for diamine oxidase activity in serum or plasma samples.
Biochem/physiol Actions
The Diamine Oxidase Assay Kit is based on the oxidation of putrescine to pyrroline plus NH3 and H2O2. The generated H2O2 is then used by HRP to oxidize a dye making it fluorescent. The increase in fluorescence at λEx = 530 nm/λEm = 585 nm is directly proportional to the enzyme activity.
Features and Benefits

• Linear detection range 0.5 to 6 U/L .
• Compatible with high-throughput handling systems.