Merck, MAK530-1KT, Alkaline Phosphatase (ALP) Assay Kit

Name: Alkaline Phosphatase (ALP) Assay Kit
Brand: Merck
SKU: MAK530-1KT

Size:1 kit, Fluorometric Alkaline Phosphatase Assay Kit, sufficient for 100 fluorometric tests

Catalog Number: MAK530-1KT

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Category

Diagnostic ELISA Kits

TagsChemistry & Biochemicals Biochemicals Metabolism Assay Kits

CollectionsMerck Biochemicals Collection: Trusted Lab Reagents & Buffers Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis.
Application
The kit is suitable for ALP activity in serum, plasma and other sources, characterization and quality control for ALP production, and for high-throughput screening for ALP modulators.
Biochem/physiol Actions
The Alkaline Phosphatase (ALP) Assay Kit utilizes 4-methylumbelliferyl phosphate that is hydrolyzed by ALP into the highly fluorescent product 4methylumbelliferone. The rate of the fluorescence increase is directly proportional to the enzyme activity.
Features and Benefits

Detection limit of 0.02 U/L
Compatible with high-throughput handling systems.

Specifications

General

NACRES	NA.84
UNSPSC Code	12161503
Quality Level	100
technique(s)	activity assay: suitable
application(s)	life science and biopharma
detection method	fluorometric
relevant disease(s)	cancer; neurological disorders; gastrointestinal diseases; genitourinary disorders
shipped in	wet ice
storage temp.	−20°C

Ordering

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