Merck, MAK562-1KT, Branched Chain Amino Acid Kit

General description
Leucine, isoleucine and valine, known collectively as branched-chain amino acids (BCAA) are unique amongst the essential amino acids in that they undergo oxidation to a greater extent in the peripheral tissues than in liver¹. Concentrations of these amino acids in plasma are altered by moderate and exhausting exercise, by diet and the nutritional status of the individual.²

In this assay, BCAA concentration is determined using a coupled enzyme reaction to catalyze the conversion of a BCAA molecule to its ketone form, which results in the conversion of NAD⁺ to NADH.³ NADH is detected by a colorimetric indicator, with an absorption maximum of 450 nm, proportional to the amount of BCAA present.
Application
BCAA Kit has been used to quantify BCAA in various types of samples, such as food, dietary supplements, blood, serum and cells.
Suitable for measuring Branched Chain Amino Acids (BCAA′s) in a variety of biological and organic samples.
Features and Benefits
Highly sensitive, simple and high-throughput assay for measuring BCAA concentration.
Detection time: only 30 minutes
Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.

Click here to download a calculator excel file