General descriptionLactate dehydrogenase catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. LDH converts pyruvate, the final product of glycolysis, to lactate when oxygen is absent or in short supply, and it performs the reverse reaction during the Cori Cycle (in the liver). At high concentrations of lactate, the enzyme exhibits feedback inhibition, and the rate of conversion of pyruvate to lactate is decreased.
Cells release LDH into the bloodstream after tissue damage or red blood cell hemolysis. As LDH is a fairly stable enzyme, its level in blood is widely used to evaluate the presence of damage and toxicity of tissue and cells.
LDH is also elevated in certain pathological conditions, such as cancer. Serum LDH is far more than a simple indicator of tumor burden; it is a complex biomarker associated with the activation of several oncogenic signaling pathways, as well as with the metabolic activity, invasiveness, and immunogenicity of many tumors. LDH constitutes an attractive target for cancer therapy. Quantification and/or inhibition of LDH, therefore, has a broad range of applications.
Three known LDH inhibitors (Oxamate, Gossypol and Quercetin) are provided as components of the kit, allowing to comparepotential novel LDH inhibitors to known ones. The LDH Inhibitor Screening Kit quantifies LDH activity and inhibition in a variety of biological samples providing quick, convenient, and highly sensitive results.
Biochem/physiol ActionsLDH Activity is based on a colorimetric method in which a colorless developer is changed to a colored product. The amount of the colorimetric (450 nm) product is proportional to the enzymatic activity present in the sample.
Unit Definition: One unit of lactate dehydrogenaseis the amount of enzyme that will oxidize 1.0 μmole of L-lactate to pyruvate per min at 37 °C.
Features and BenefitsCompatibility with High-Throughput Systems: Seamlessly integrate our kit into high-throughput handling systems, ensuring smooth operations and accurate results, enhancing efficiency in your laboratory workflow.
Convenient Data Analysis: Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.
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