Merck, MAK606-1KT, Total Iron Assay Kit

General description
Increased concentrations of iron in the blood can indicate several conditions, including red blood cell destruction, decreased red blood cell survival, acute hepatitis, and certain sideroblastic anemias. Elevated iron levels may also result from excessive dietary intake of iron-rich foods or defects in iron storage, such as in cases of pernicious anemia. Conversely, decreased blood iron levels can arise from inadequate dietary iron intake, chronic blood loss due to conditions like gastrointestinal bleeding or heavy menstruation, and increased physiological demands, particularly during pregnancy when iron requirements are elevated.
Application
The kit is suitable for the measurement of iron in various biological samples, including blood, serum, tissues and cells.
Biochem/physiol Actions
The Total Iron Assay Kit is suitable for quantitively measuring low concentrations of ferrous iron (50-1000 nanograms). The assay is based on measuring the absorbance of the iron probe (absorbing at 593 nm) that is formed in the reaction. The kit directly measures ferrous iron (Fe²⁺) which binds to the probe to give a stable deep blue color. To measure ferric iron (Fe³⁺), the ion needs to be reduced to Fe²⁺ with the supplied reducer, which can then be detected by the probe. Enabling the distinguishment between ferric and ferrous iron. In serum, there may be contamination of the sample with copper ions which can also react with the reagent, therefore thiourea is added to the assay buffer.
Features and Benefits
Compatibility with High-Throughput Systems: Seamlessly integrate our kit into high-throughput handling systems, ensuring smooth operations and accurate results, enhancing efficiency in your laboratory workflow.

Convenient Data Analysis: Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.

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