Merck, MAK607-1KT, Acetoacetate Assay Kit

Name: Acetoacetate Assay Kit
Brand: Merck
SKU: MAK607-1KT

Size:, AAT Assay Kit, sufficient for 100 colorimetric tests

Catalog Number: MAK607-1KT

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Category

BCA Protein Assay Kits

TagsChemistry & Biochemicals Biochemicals Metabolism Assay Kits

CollectionsMerck Biochemicals Collection: Trusted Lab Reagents & Buffers Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Acetoacetate is one of three ketone bodies produced in the liver mainly from oxidation of fatty acids and is normally present at low concentrations in urine and blood. Increased ketone concentrations in the blood may lead to metabolic acidosis, which has been associated with diabetes, childhood hypoglycemia, growth hormone deficiency, alcohol orsalicylate intoxication, and inborn errors of metabolism. Acetoacetate is an unstable ketone body that spontaneously decarboxylates to release carbondioxide and acetone.
Application
The assay is sensitive and accurate, with a linear detection range of 0.012 to 1 mM (1.2–100 nmol/well).

Suitable for the direct assay of serum, plasma, urine, and other biological samples.
Biochem/physiol Actions
The Acetoacetate Assay Kit isbased on a reaction with sodium nitroprusside forming a product with absorbanceat 550 nm, enabling simple, direct, and automation-ready measurement ofacetoacetate in a 96-well format.

Specifications

General

UNSPSC Code	41116104
usage	sufficient for 100 colorimetric tests
detection method	colorimetric
relevant disease(s)	diabetes; neonatal diseases; endocrinological disorders
storage temp.	−20°C

Ordering

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