Merck, MAK615-1KT, Pepsin Inhibitor Assay Kit

General description
Pepsin (EC3.4.23.1) is a digestive, serine protease that hydrolyzes dietary proteins inmany eukaryotic and prokaryotic organisms. Pepsin predominantly cleaves peptidechains at the amino side of the aromatic phenylalanine, tryptophan, andtyrosine amino acids.
Application
For the evaluation of drugs and screening of potential inhibitors to pepsin proteases.
Biochem/physiol Actions
The PepsinInhibitor Assay Kit uses a fluorescein isothiocyanate (FITC)-labeled syntheticsubstrate. The fluorescein label is highly quenched. Upon digestion by pepsinpresent in the sample, the substrate is cleaved into smaller peptides, whichabolishes the quenching of the fluorescence label. The fluorescence orfluorescence polarization (FP) of the FITC-labeled fragments is measured atλex/em = 485/530 nm. Inhibition is determined by the decrease in fluorescence.