Merck, MAK616-1KT, Phytase Assay Kit

General description
Phytase catalyzesthe hydrolysis of phosphoester bonds on myoinositol‑(1,2,3,4,5,6)-hexakisphosphate(Phytic acid or IP6) thereby releasing inositol and phosphate. Phytic acid is amajor storage reservoir for phosphate in plants. Phytase is abundant in grainssuch as wheat and barley, and the hydrolysis of phytic acid by single-stomachedanimals is a crucial aspect of animal nutrition. In addition, the lack ofphytase in single-stomached animals can lead to excessive phosphorus leachinginto the environment due to undigested phytic acid.
Biochem/physiol Actions
The color intensity, measured at 620 nm, is proportionate to the amount of phosphate released from phytic acid. The detection range is 0.01 to 20 U/L phytase in a 96-well plate assay.