Merck, MAK620-1KT, Trypsin Inhibitor Assay Kit

General description
Trypsin (EC3.4.21.4) is a digestive, serine protease that hydrolyzes dietary proteins inmany eukaryotic and prokaryotic organisms. Trypsin predominantly cleavespeptide chains at the carboxyl side of lysine and arginine amino acids, but notbefore proline.
Application
Suitable for evaluation of drugs and screening of potential inhibitors to trypsinproteases.
Biochem/physiol Actions
The TrypsinInhibitor Assay Kit uses a fluorescein isothiocyanate (FITC)-labeled syntheticsubstrate. The fluorescein label is highly quenched. Upon digestion by trypsinpresent in the sample, the substrate is cleaved into smaller peptides, whichabolishes the quenching of the fluorescence label. The fluorescence orfluorescence polarization (FP) of the FITC-labeled fragments is measured atλex/em = 485/530 nm. Inhibition is determined by the decrease in fluorescence.