Merck, MAK622-1KT, Fatty Acyl-CoA Assay Kit

General description
FattyAcyl-CoAs are a combination of Coenzyme A covalently bound to long chain fattyacids. Fatty acyl-CoAs serve as both a precursor for triglyceride andphospholipids synthesis, and a product of lipid catabolism. Catabolically, theyfacilitate the transport of fatty acids to the mitochondria for shuttling viathe carnitine shuttle and subsequent β‑oxidation of the fatty acid. Conversionof fatty acids to fatty acyl-CoAs is necessary for generation of ATP duringperiods of high energy demand. Elevated fatty acyl-CoA levels may indicatedysregulation of lipid metabolism due to insulin resistance, for example.Certain genetic disorders may also affect fatty acyl-CoA levels.
Application
Suitable for the quantitative determination of fatty acyl-CoAs in tissue and cell lysates.
Biochem/physiol Actions
The Fatty Acyl‑CoAassay works using a combination of enzymes that utilize fatty acyl-CoA as asubstrate, which is coupled to form a fluorescent product. The resultingfluorescence intensity at λex/em = 530/585 nm is linear to the fatty acyl-CoAconcentration in the sample.