Merck, MAK625-1KT, HDL and LDL/VLDL Assay Kit

General description
Cholesterolconcentrations in High-Density Lipoprotein (HDL) and Low-Density(LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronaryheart disease. Functional HDL offers protection by removing cholesterol fromcells and atheroma. Higher concentrations of LDL and lower concentrations offunctional HDL are strongly associated with cardiovascular disease due tohigher risk of atherosclerosis. The balances between high- and low-densitylipoproteins are solely genetically determined, but can be changed bymedications, food choices and other factors.
Application
Suitable for the quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism.
Biochem/physiol Actions
HDL andLDL/VLDL Assay Kit is based on an improved PEG precipitation method in whichHDL and LDL/VLDL are separated, and cholesterol concentrations are determinedusing cholesterol esterase/cholesterol dehydrogenase reagent. In this reaction,NAD is reduced to NADH. The optical density of the formed NADH at 340 nm isdirectly proportionate to the cholesterol concentration in the sample. Thisassay has a detection limit of 5 mg/dL with linearity up to 300 mg/dLcholesterol.