Merck, MAK636-1KT, MMP-1 Inhibitor Assay Kit

General description
Matrixmetalloproteinase-1 or MMP-1 (EC 3.4.24.7) is a Ca²⁺ - and Zn²⁺-dependent,interstitial collagenase that plays a role in the remodeling of theextracellular matrix of tissues. MMP-1 is up-regulated in metastatic cancercells and in rheumatoid arthritis.
Application
Suitable for the evaluation of drugs and screening of potential inhibitors to MMP-1.
Biochem/physiol Actions
TheMMP-1 Inhibitor Assay Kit uses a quenched synthetic substrate. Upon digestionby MMP-1, the substrate is cleaved, which abolishes the quenching of thefluorescence label. The fluorescent fragment is measured at λex/em = 490/520nm. Inhibition is determined by the decrease in fluorescence.