Merck, MAK637-1KT, MMP-9 Inhibitor Assay Kit

General description
Matrixmetalloproteinase-9 or MMP-9 (EC 3.4.24.35) is a Ca²⁺ - and Zn²⁺-dependent,interstitial gelatinase that plays a role in the remodeling of theextracellular matrix of tissues. MMP-9 plays a central role in processesdiverse as wound healing, angiogenesis, and bone development. In addition toits role in normal growth and development, MMP-9 is up-regulated in manydiseases, including metastatic cancer, rheumatoid arthritis, and asthma.
Application
Suitable for the evaluation of drugs and screening of potential inhibitors to MMP-9.
Biochem/physiol Actions
TheMMP-9 Inhibitor Assay Kit uses a quenched synthetic substrate. Upon digestionby MMP-9, the substrate is cleaved, which abolishes the quenching of thefluorescence label. The fluorescent fragment is measured at λex/em = 490/520nm. Inhibition is determined by the decrease in fluorescence.