Merck, MAK639-1KT, Peroxide Assay Kit

General description
Peroxide(hydrogen peroxide H₂O₂) is one of the key reactiveoxygen species formed under oxidative stress conditions. High levels ofperoxide formation have been linked to pathological conditions such as aging,asthma, diabetes, atherosclerosis, cataracts, inflammatory arthritis andneurodegenerative diseases.
Application
Suitable for the direct assay of H₂O₂ and organic peroxides in biological samples (e.g. serum, citrate-plasma, urine, cell lysate, culture medium) and the determination of the effects of drugs on peroxide metabolism.
Biochem/physiol Actions
The improvedperoxide assay kit is designed to measure peroxide concentration in biologicalsamples without any pretreatment. This method utilizes the chromogenic Fe³⁺‑xylenolorange reaction, in which a purple complex is formed when Fe²⁺provided in the reagent is oxidized to Fe³⁺ by peroxides present inthe sample. The intensity of the color, measured at 540‑610 nm, is an accuratemeasure of the peroxide level in the sample. The optimized formulationsubstantially reduces interference by substances in the raw samples.

This assayhas a detection limit of 0.54 µM (18.4 ng/mL) and linear up to 30 µM (1,020ng/mL) H₂O₂ in 96-well plate assay.