General description
Mouse cytokines are essential signaling proteins that regulate immune responses, inflammation, and intercellular communication in murine models, serving as critical biomarkers for understanding disease mechanisms, drug efficacy, and therapeutic safety in preclinical research. These small regulatory molecules, including interleukins, interferons, tumor necrosis factors, and growth factors, control fundamental biological processes such as T-cell activation, macrophage polarization, antibody production, and tissue repair, making them invaluable for studying cancer immunotherapy, autoimmune diseases, and infectious disease responses. Multiplex detection of mouse cytokines enables researchers to simultaneously analyze complex immune networks with high sensitivity and minimal sample consumption, providing comprehensive insights that are essential for translating laboratory findings into clinical applications due to the strong evolutionary conservation of cytokine pathways between mice and humans.
Application
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 68 analytes in mouse serum, plasma, and cell culture supernatants.
Analytes included: BAFF, Betacellulin, CHI3L1, CXCL16, EGF, Eotaxin/CCL11, Erythropoietin, Exodus-2/CCL21, FGF-2, FLT3L, Fractalkine/CX3CL1, G-CSF, GDF-15/MIC-1, GM-CSF, Granzyme B, IFNα, IFNβ, IFNγ, IL-10, IL-11, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-16, IL-17A, IL-17F, IL-18, IL-1α, IL-1β, IL-2, IL-20, IL-22, IL-28B/IFNλ3, IL-3, IL-31, IL-33/NF-HEV (mature), IL-4, IL-5, IL-6, IL-7, IL-9, IP-10/CXCL10, KC/GROα/CXCL1, LIF, LIX/CXCL5, MCP-1/CCL2, MCP-2/CCL8, MCP-3/CCL7, MCP-5/CCL12, M-CSF, MDC/CCL22, MIG/CXCL9, MIP-1α/CCL3, MIP-1β/CCL4, MIP-2/CXCL2, MIP-3α/CCL20, MIP-3β/CCL19, RANTES/CCL5, sCD137/4-1BB/TNFRSF9, sFas/TNFRSF6, sFasL, sICAM-1, sRAGE, TARC/CCL17, TECK/CCL25, TNFα, and VEGF-A.
The following analytes in this kit are notcompatible with the MAGPIX® System: sFasL, IL-16, sFas, IL-20, sCD137, sICAM-1, CXCL16, sRAGE, CCL25/TECK, BAFF, GDF-15, FLT3L, CHI3L1, MCP-2, MCP-3, EPO, Fracktalkine, IL-31, and Betacellulin
Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.
Features and Benefits
Largest and Most Comprehensive Mouse Cytokine Assay Available: Offers an expansive selection of analytes across key immune and inflammatory pathways, enabling simultaneous detection of dozens of cytokines, chemokines, and growth factors in a single sample.
Fully Configurable Multiplex Assay: Design your own panel by selecting up to 68 analytes, enabling tailored cytokine and chemokine profiling that meets your specific research needs in biomarke discovery.
Efficient Sample Management: Analyze multiple targets with just 25 µL/well (1:2 diluted), conserving samples and maximizing data from limited material.
Adaptable incubation options: Opt for same-day or overnight incubation to seamlessly integrate into your workflow, enhancing productivity.
Consistent and Reliable Data: Experience proven lot-to-lot consistency for trustworthy results, vital for regulatory compliance and reproducibility in analytical R&D.
Streamlined Assay Setup: Ready-to-use reagents and comprehensive kit components, including Serum Matrix and Quality Controls, simplify preparation and reduce the risk of errors.
Legal Information
Luminex is a registered trademark of Luminex Corporation
MAGPIX is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
For research use only. Not for use in diagnostic procedures.
Label License/Sticker for Assay Product:
By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
