Description
General description
Found only in mammals, Immunoglobulin E (IgE) plays an essential role in allergic reactions and is predominantly associated with type 1 hypersensitivity. IgE is the least abundant immunoglobulin isotype in normal or topic mammals, present at only 0.05% of IgG serum concentrations. It can trigger robust immune reactions, with up to 10 times the typical level detected in the serum of atopic mammals, although increased levels occur only in approximately 50% of mammals with allergic disorders. IgE binds with high affinity to FcRI, found primarily on mast cells and basophils, priming the cells to recognize specific antigens. Once primed and with repeated exposure to the same antigen, these cells release molecules such as histamines, prostaglandins, and leukotrienes, resulting in an “allergic reaction”.
Application
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Analyze IgE in mouse serum, plasma, tissue/cell lysates, and cell culture supernatants.
Analytes included: IgE
Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.
Features and Benefits
Targeted IgE Detection: Specifically measures mouse IgE, the immunoglobulin central to allergic reactions and type I hypersensitivity.
Ultra-Low Sample Volume: Requires only 2 µL of sample—ideal for studies with limited material or small animal models.
Versatile Sample Compatibility: Verified for serum, plasma, tissue/cell lysates, and culture supernatants, offering flexibility across experimental designs.
High Sensitivity and Specificity: Detects low levels of IgE, with negligible cross-reactivity to other immunoglobulin isotypes.
Fast Assay Completion: Full assay can be completed in one day, accelerating time-to-results.
Support Allergy and Immunology Research: Enables detailed profiling of atopic conditions, immune dysregulation, and IgE-related disease states.
Legal Information
Luminex is a registered trademark of Luminex Corporation
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
For research use only. Not for use in diagnostic procedures.
Label License/Sticker for Assay Product:
By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
