Merck, MHA000P2E, m-Endo Total Coliform Broth

General description
m-Endo Total Coliform Broth is a selective and differential medium used to estimate the coliform bacteria in water samples by membrane filtration technique. The medium enables differentiation between lactose fermenters and non-fermenters. The use of sodium sulfite and basic fuchsin instead of bile salts inhibits the growth of Gram-positive bacteria. Lactose fermenting bacteria produce acetaldehyde that reacts with the sodium sulfite and fuchsin to form red colonies. The rapid fermentation of lactose by the organisms produces aldehydes, which leads to the development of a metallic sheen. Lactose non-fermenters form colorless colonies.
Application
m-Endo Total Coliform Broth is suitable for Total coliform detection in water samples. The media enables to differentiate between lactose fermenters from lactose non-fermenters.
Features and Benefits
Ready-to-use Liquid ampoule media solve numerous problems in microbiology laboratories where time, equipment, and trained personnel are often in short supply.
They are available in convenient, single-test 2 mL plastic ampoules, eliminating the manipulation of glass ampoules and avoiding the waste and spoilage associated with bulk packaged media.

Incubation Temperature: 35 °C ± 0.5
Incubation Time: 24 hrs ± 2
Standard Methods for the Examination of Water and Wastewater
Analysis Note
Coliform colonies appear deep red with distinct green metallic sheen
QC organisms
E. coli (ATCC 25922)

Mixed culture (E. coli, P. aeruginosa and P. vulgaris)
Target Microorganisms: Total coliforms
Legal Information
EZ-FIT is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany