Merck, MOD50-1KT, Imprint® DNA Modification Kit

General description
The Imprint® DNA Modification Kit contains all of the reagents necessary for complete bisulfite conversion and subsequent purification of DNA samples. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. Converted DNA is suitable for a variety of downstream applications including Methylation-Specifc PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.
Application
Imprint® DNA Modification Kit has been used for bisulfite modification of DNA.
Features and Benefits
Only 50 picograms of DNA or 20 cells are required
Procedure takes less than 2 hours
Greater than 99% conversion rate
Extremely low degradation
Option of convenient one-step protocol
Consistent and reproducible Bisulfite Modification
Can be used with genomic, endonuclease digested, and FFPE DNA

Preparation Note
All components can be stored at room temperature. Each vial of DNA Modification Powder is sufficient for ten DNA modifications. Once dissolved, the solution can be stored at -20 °C for one week, kept away from light. Before use, the frozen solution must be thawed at room temperature and vortexed for two minutes.
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany