Merck, MPE005025, Microcon® Centrifugal Filters

Microcon MPE005025 centrifugal filter is used to concentrate and desalt biomolecule solutions with a nominal molecular-weight limit of 5 kDa. The device processes up to 0.5 mL in a centrifuge that accepts 1.5 mL tubes and can achieve concentration factors up to 100-fold. A reverse-spin step transfers the concentrate into a standard microcentrifuge tube and maximizes recovery from small samples. The 0.32 cm² Biomax polyethersulfone membrane provides the ultrafiltration surface, while the assembly uses a polyacetal membrane-support base, polycarbonate upper device, polypropylene filtrate/concentrate tube, and medical-grade silicone O-ring. The device measures 4.5 cm long and 12.3 mm in diameter. More than 10 µL remains as hold-up volume, and the product is supplied non-sterile. This format supports laboratories that need to reduce sample volume, exchange buffer, or remove low-molecular-weight solutes before downstream analysis. The compact centrifugal workflow avoids a separate pressure-driven filtration system and keeps both filtrate and concentrate in standard microfuge-compatible tubes for convenient retention or storage. The Biomax membrane and reverse-spin recovery function enable fast processing and high recovery from limited-volume samples, particularly at concentration factors below 20-fold. MPE005025 is supplied as 25 devices. Its 5 kDa cutoff, 0.5 mL capacity, small membrane area, and recovery-focused design suit reproducible concentration and desalting of small biomolecular samples.