General descriptionHAVCR1 is a trans-membrane glycoprotein that is highly expressed in kidney proximal tubular cells with kidney injury. The KIM-1 ectodomain is cleaved, released into urine, and canbe quantified as a sensitive and specific biomarker for Acute Kidney Injury (AKI) in rodents and humans. In a recent study it was demonstrated that HAVCR1 is released into the circulation where it reports kidney injury in mice, rats, and humans with AKI and chronic kidney disease (CKD). Urinary HAVCR1 and MCP-1 levels, either alone or in combination, were significantly higher than those in AKI negative patients. Importantly, the combination of HAVCR1 and MCP-1 outperformed either biomarker alone.
ImmunogenSVKVGGEAGPSVTLPCHYSGAVTSMCWNRGSCSLFTCQNGIVWTNGTHVTYRKDTRYKLLGDLSRRDVSLTIENTAVSDSGVYCCRVEHRGWFNDMKITVSLEIVPPKVTTTPIVTTVPTVTTVRTSTTVPTTTTVPTTTVPTTMSIPTTTTVLTTMTVSTTTSVPTTTSIPTTTSVPVTTTVSTFVPPMPLPRQNHEPVATSPSSPQPAETHPTTLQGAIRREPTSSPLYSYTTDGNDTVTESSDGLWNNNQTQLFLEHSLLTANTTKGDYKDDDDKGHHHHHHHHGGQ
Biochem/physiol ActionsSILu™Prot HAVCR1 is a recombinant, stable isotope-labeled human HAVCR1 which incorporates [¹³C₆, ¹⁵N₄]-Arginine and [¹³C₆, ¹⁵N₂]-Lysine. Expressed in human 293 cells, it is designed to be used as an internal standard for bioanalysis of HAVCR1 in mass-spectrometry. SILu Prot HAVCR1 is a protein consisting of 290 amino acids (the extracellular domain: Ser21-Gly290, and including C-terminal polyhistidine and FLAG® tags), with a calculated molecular mass of 31 kDa.
Physical formSupplied as a lyophilized powder containing phosphate buffered saline.
Legal InformationThis product is licensed under U.S. Patent No. 7,396,688 and foreign counterparts from E. I. du Pont de Nemours and Company. The purchase of this product conveys to the buyer the nontransferable right to use the purchased amount of the product for research and development only, including services for a third party for consideration. The buyer cannot sell or otherwise transfer this product, its components or materials made using this product or its components to a third party. Information about licenses for excluded uses is available from: E. I. du Pont de Nemours and Company; Attn: Associate Director, Commercial Development; DuPont Experimental Station E268; 200 Powdermill Rd.; Wilmington, DE 19803; 1-877-881-9787 (voice), 1-302-695-1437 (fax),
[email protected].
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
SILu is a trademark of Sigma-Aldrich Co. LLC
