Description
General description
Gel filtration chromatography is a well-known technique for measuring the size and molecular weight of proteins. It separates proteins based on their ability to diffuse into the pores of the resin. Larger proteins have more difficulty entering the resin pores, so they flow through the column more quickly than smaller proteins. As a result, the proteins are eluted from the column in order of decreasing molecular weight.
Application
Gel Filtration Markers Kit for Protein Molecular Weights 29,000-700,000 Da has been used:
for protein purification and analyses (gel-filtration experiments)
for size-exclusion chromatography/gel filtration chromatography of synaptoneurosome extract
for glycerol gradient centrifugation
to study glucocorticoid-induced lymphocytolysis as a model system for apoptosis within the immune system
to isolate Polygonatum odoratum lectin, which showed remarkable anti-HSV-II activity towards Vero cells, cytotoxicity towards human melanoma A375 cells and induced apoptosis in a caspase-dependent manner
Biochem/physiol Actions
The method described in the bulletin for determining molecular masses using gel filtration chromatography is a modified version of existing published techniques. The protein standards included in this kit may be compatible with other chromatographic systems like high-performance liquid chromatography (HPLC). However, certain buffer systems may affect the elution volumes of albumin and carbonic anhydrase. The proteins in this kit have a molecular mass range spanning from 29 kDa to 699 kDa.
Other Notes
For research use only. Not for drug, household, or other uses.
Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Please refer to the product information sheet to learn more about reagents, storage and stability, and procedure.
