General descriptionThis labeling kit is for labeling 50-100 μg antibody with the green fluorescent dye CF488A. Mix-n-Stain CF dye antibody labeling kits are a revolutionary antibody labeling technology that permits one to label antibodies with our superior CF dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. CF488A is a green fluorescent dye optimally excitable by the 488 nm argon laser line. Under common detection settings, CF488A is similar to Alexa Fluor™ 488 in brightness and photostability. In microscopy or flow cytometry applications using a 520/20 filter, CF488A may appear brighter than Alexa Fluor™ 488 due to its shorter emission wavelength, which makes the dye optically more compatible. The shorter wavelength of CF488A also minimizes the dye′s spill-over into longer wavelength channels, making the dye advantageous in multi-color detection. Like Alexa Fluor™ 488, CF488A is highly water soluble, but is not as excessively negatively charged. As a result, antibody conjugates prepared from CF488A retain excellent specificity to ensure high signal-to-noise ratio.
ApplicationMix-n-Stain™ CF™ 488A Antibody Labeling Kit (50-100μg) has been used in antibody conjugation and to conjugate pSMAD5 and Nkx6.1.
Features and BenefitsMix-n-Stain CF dye antibody labeling kits dramatically simplify the process of preparing fluorescently labeled antibodies, particularly primary antibodies. Simply mix your antibody with a CF dye of your choice in the buffer provided, a step that takes less than 30 seconds of hands-on time. After waiting for another 30 minutes, without a separation step, you will have a fluorescent antibody conjugate that is as good as the best pre-labeled fluorescent antibody you can buy from some of the leading suppliers. There is no need to calculate how much dye you should use; just mix your antibody with the entire amount of dye provided and you will always produce optimal labeling. Moreover, the labeling reaction can tolerate the presence of common stabilizers, such as sodium azide, Tris, glycerol and low level of BSA.
Analysis NoteDirect replacement for: Alexa Fluor™ 488, Cy®2, DY-495, DyLight®488, FAM, FITC, Fluorescein
Other NotesThe kit consists of 1 vial each of Mix-n-Stain Dye, Mix-n-Stain Reaction Buffer (10x), Mix-n-Stain Storage Buffer, and an ultrafiltration vial.
Legal InformationThis product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email:
[email protected].
ALEXA FLUOR is a trademark of Life Technologies
CF is a trademark of Biotium, Inc.
Cy is a registered trademark of Cytiva
DyLight is a registered trademark of Pierce Biotechnology, Inc.
Mix-n-Stain is a trademark of Biotium, Inc.
DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
