Merck, N1628-25G, Novobiocin sodium salt

General description
Novobiocin sodium salt is an antibiotic derived from soil bacteria. Novobiocin inhibits the action of bacterial DNA gyrase and belongs to a class of compounds called coumarin antibiotics. Coumarins are heterocyclic compounds with a wide range of biological activity, including antibacterial, anticoagulant, antioxidant, anti-inflammatory, antiviral, antitumor, and enzyme inhibitory effects.
Application
For the production of positively supercoiled plasmid DNA. Inhibitor of bacterial DNA gyrase and eukaryotic DNA topoisomerase. Inhibitor of retrovirus RNA-dependent DNA-polymerase.
Novobiocin Sodium Salt, an inhibitor of Hsp90, can be used:

• in the research heat shock protein inhibition and to produce positively supercoiled plasmid DNA
• in the research on the efficacy of probiotics
• in the research on the efficacy of human monocytes and dendritic cells for T-cell replication

Biochem/physiol Actions
Mode of Action: Inhibits DNA synthesis by inhibiting the enzyme Topoisomerase II.
Antimicrobial spectrum: Gram-positive bacterial.
Novobiocin inhibits DNA synthesis by inhibiting the GyrB subunit of the Topoisomerase enzyme involved in energy transduction. Novobiocin acts as a competitive inhibitor of the ATPase reaction catalysed by GyrB.
Features and Benefits
High quality antibiotic suitable for mulitple research applications
Preparation Note
Stored in frozen aliquots, Keep container tightly closed in a dry and well-ventilated place. Light sensitive
Other Notes
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