Merck, N2635-100UN, Nucleoside 5′-Diphosphate Kinase from bovine liver

General description
Research area: Cell Signaling

Nucleoside 5′-Diphosphate Kinase (NDK) is a ubiquitous housekeeping enzyme. Nucleoside Diphosphate Kinase exists in two isoforms in eukaryotic cells, NDK-A and NDK-B. These enzymes are found expressed both in the mitochondria and the cytoplasm.
Application
Nucleoside 5′-Diphosphate Kinase has been used:nucleoside diphosphate kinase (NDPK) assays
coupled NDPK-luciferase assay to determine the amounts of Ras-bound guanosine triphosphate (GTP)
non-metastatic protein 23 (NM23) growth stimulation assay
in a study to assess inhibition of type I Fc epsilon receptor mediated Ca²⁺ influx and mediator secretion in rat mucosal mast cells
in a study to investigate protein synthesis in rabbit reticulocytes

Biochem/physiol Actions
Nucleoside 5′-Diphosphate Kinases (NDKs) aid in the synthesis of nucleoside triphosphates (NTPs) by transferring a phosphate group from ATP to nucleoside diphosphates (NDPs). NDKs provide NTPs for nucleic acid synthesis, cytidine 5′-triphosphate (CTP) for lipid synthesis, uridine 5′-triphosphate (UTP) for polysaccharide synthesis, and guanosine triphosphate (GTP) for protein elongation, signal transduction, and microtubule polymerization.
Other Notes
One unit will convert 1.0 μmole each of TDP and ATP to TTP and ADP per min at pH 7.6 at 25 °C in a coupled system with PK/LDH.