Description
General description
Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.
Application
4-Nitrophenyl phosphate disodium salt hexahydrate has been used:
as a substrate for alkaline phosphatase coupled anti-rabbit antibody in enzyme-linked immunosorbent assay (ELISA)
as a substrate for the mouse secondary antibody linked to alkaline phosphatase in immunoenzymometric assay
in ELISA to analyze the presence of antibodies to Pseudomonas aeruginosa in animal serum sample
Preparation Note
Contains 20 mg substrate per tablet