Merck, NA0200-1KT, GenElute™ HP Plasmid Midiprep Kit

General description
The GenElute HP Plasmid Midiprep Purification kits offer an ultrafast and efficient solution for large-scale plasmid preparation from E. coli cultures. These kits combine silica-based membrane technology and the convenience of spin or vacuum format. The kits also include a special filter cartridge, which replaces the centrifugation step following alkaline lysis. Following lysis the DNA is bound to the silica membrane and contaminants are removed with a simple wash step. The DNA is then eluted in water or TE buffer by centrifugation.

The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
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Application
GenElute™ HP Plasmid Midiprep Kit has been used to isolate and purify plasmids.
Features and Benefits
From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
Up to 350 μg of high-copy plasmid DNA
Flexibility of a vacuum or spin format
No phenol/chloroform extraction or alcohol precipitation required
Contains fewer plastic components, reducing the amount of waste

Other Notes
For additional information, please see www.sigma-aldrich.com/genelutehp.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC