Description
General description
Click here to view the kit protocol
The GenElute Blood Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from fresh or aged (older than 24 hours) whole blood. The kit combines the advantages of silica binding with a microspin format, and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.
Application
GenElute™ Blood Genomic DNA Kit has been used:
to extract DNA from samples of large granules (LG) and smaller granules (SG) obtained from vasectomized rabbits
to isolate DNA from the blood samples
to extract whole blood DNA from the left blood cells
The purified genomic DNA is ready for downstream applications such as:
restriction endonuclease digestions
PCR
Southern blots
sequencing reactions
cloning
Biochem/physiol Actions
The starting material is lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. A Prewash Solution is provided to help remove contaminants that are associated with aged (older than 24 hours) whole blood samples. After washing to remove contaminants, the DNA is eluted in 200 mL of a Tris-EDTA solution.
The expected yields of genomic DNA will vary depending on the amount and nature of the starting material used (for example, 4 to 10 μg of RNase A-treated DNA can be isolated from 200 μl of fresh whole blood in less than one hour). DNA purified with this kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.
Features and Benefits
Starting material: Up to 200 μl of fresh or aged blood
Expected yield: Up to 10 μg
Elution volume: 400 μl
Time required: A260/A280 ratio: 1.6 - 1.9
Compatible with many anticoagulants, including EDTA, Heparin, and Sodium Citrate
Typical yields 4-10 μg from 200 μL of whole blood
Pure genomic DNA with a A260/A280 ratio between 1.6 and 1.9
Isolate DNA up to 50 kb in length
Other Notes
For additional information, please see www.sigma-aldrich.com/genomicdna.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
