Merck, NA46-100UG, Anti-p300 Mouse mAb (NM-11)

General description
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the p300 protein.
Recognizes the p300 protein in A431, HeLa, and ML-1 cells.
This Anti-p300 Mouse mAb (NM-11) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of p300.
Immunogen
Epitope: within amino acids 2071-2091 near the CBP C-terminus
Human
recombinant, human p300 protein
Application
Frozen Sections (see application references)
Immunoblotting (1 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1 µg/sample)
Paraffin Sections (1-5 µg/ml, heat pre-treatment required)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 0.05 M sodium phosphate buffer, 50% glycerol.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
A431, HeLa, or ML-1 cells
Other Notes
Antibody should be titrated for optimal results in individual systems.
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Legal Information
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Toxicity: Standard Handling (A)