Merck, NB20-100UG, Anti-AnkyrinG Mouse mAb (463)

General description
Anti-Ankyrin G, mouse monoclonal, clone 463, recognizes the ~190 kDa ankyrin G protein in brain extracts. It is validated for Western blotting, immunofluorescence, and frozen sections.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63/Ag 8.653 mouse myeloma cells. Recognizes the ~190 kDa isoform of AnkyrinG.
Recognizes the ~190 kDa ankyrinG protein in brain extracts. Reacts with all spliced forms of ankyrinG.Antibody Target Gene Symbol: ANK3
Target Synonym: 2900054D09RIK, AI314020, AnkG, Ankyrin-3, ANKYRIN-G, FLJ45464, MGC14049
Entrez Gene Name: ankyrin 3, node of Ranvier (ankyrin G)
Hu Entrez ID: 288 (Related Antibodies: AP1034, PC523, PC522, AP1057)
Mu Entrez ID: 11735
Rat Entrez ID: 361833

Immunogen
Human
a synthetic peptide corresponding to amino acids from the spectrin-binding domain of human ankyrinG
Application

Frozen Sections (1-2 g/ml)
Immunoblotting (2-5 g/ml)
Immunofluorescence (1-2 g/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Analysis Note
Positive Control
Mouse or rat brain extract or unmyelinated axons
Other Notes
AnkyrinG is a good immunohistochemical marker for axon initial segments, nodes of Ranvier, and epithelial cell-cell contact sites. Antibody should be titrated for optimal results in individual systems.
Davis, J.Q., et al. 1995. Mol. Biol. Cell6, 177a, supplement.
Kunimoto, M., et al. 1995. FEBS Lett.357, 217.
Lambert, S., et al. 1995. Mol. Biol. Cell6, 98a supplement.
Bennett, V. and Gilligan, D.M. 1993. Ann. Rev. Cell Biol.9, 27.
Kunimoto, M., et al. 1991. J. Cell Biol.115, 1319.
Otto, E., et al. 1991. J. Cell Biol.114, 241.
Davis, J., and Bennett, V. 1984. J. Biol. Chem.259, 13550.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)