Merck, NE1015-100UL, Anti-Glial Fibrillary Acidic Protein Cocktail Mouse mAb (SMI-22)

General description
Mouse monoclonal antibody cocktail that contains a mixture of 3 antibodies supplied as undiluted ascites. Recognizes the ~50 kDa glial fibrillary acidic protein.
Recognizes ~50 kDa glial fibrillary acidic protein (GFAP) in human and bovine cytoskeletal preparations.
This Anti-Glial Fibrillary Acidic Protein Cocktail Mouse mAb is validated for use in ELISA, Frozen Sections, WB, ICC, Paraffin Sections for the detection of Glial Fibrillary Acidic Protein.
Immunogen
Bovine
purified bovine GFAP protein
Application

ELISA (1:1000)
Frozen Sections (1:1000, see comments)
Immunoblotting (1:1000)
Immunocytochemistry (1:1000, see comments)
Paraffin Sections (1:1000, trypsin or heat pre-treatment required)
Physical form
Undiluted ascites.
Preparation Note
Upon initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Astrocytes or cytoskeletal preparations
Other Notes
This cocktail is derived from the Bigner-Eng clones MAb1B4, MAb2E1, and MAb4A11 and provides a means for more comprehensive detection of astrocytomas than each clone alone. Each component is specific for GFAP and stains astrocytes and astrocytic processes as well as Bergman glia. Recognizes both anaplastic and reactive astrocytes by immunocytochemical staining. Does not recognize metastatic tumors and brain tumors of non-astrocytic origin, including medulloblastomas, meningiomas, choroid plexus papillomas, and schwannomas. For staining paraffin sections it is recommended that de-paraffinized sections be treated with 0.1% trypsin in 50 mM Tris-HCl, pH 7.6 for 20-30 min at 37°C or boiled in Tris-buffered saline, pH 9.0 for 15 min to expose the epitope. For immunocytochemistry or staining frozen sections, post-fixation in cold methanol or methanol/hydrogen peroxide for 10 min is required for access to the astrocytes in the sample. Antibody should be titrated for optimal results in individual systems.
Vick, W.W., et al. 1987. Acta. Cytol.31, 816.
McLendon R.E., et al. 1986. J. Neuropathol. Exp. Neurol.45, 692.
Pegram, C.N., et al. 1985. Neurochem. Pathol.3, 119.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)